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precisionxtm multiplex grna cloning kit  (System Biosciences Inc)

 
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    System Biosciences Inc precisionxtm multiplex grna cloning kit
    Precisionxtm Multiplex Grna Cloning Kit, supplied by System Biosciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/precisionxtm multiplex grna cloning kit/product/System Biosciences Inc
    Average 90 stars, based on 1 article reviews
    precisionxtm multiplex grna cloning kit - by Bioz Stars, 2026-04
    90/100 stars

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    (A) RNAPII ChIA-PET linkages at the MYC locus. ChIP-seq tracks were shown on the top. MYCSE1 (-525kb from TSS) and MYCSE2 (-428kb from TSS) were indicated by red box below the tracks. RNAPII ChIA-PET links were shown by magenta lines. Orange boxes indicated the regions targeted by CRISPR. (B) Validation of MYCSE1 Deletion. Cas9 stable LCLs or BJAB were transduced with paired <t>gRNA</t> targeting the edges of ESE. After puromycin selection, genomic DNA was prepared from LCLs and BJAB cells transduced with dual gRNA. The targeted region was PCR amplified. The presence or absence of the MYC SE1 was shown. (C) MYCSE1 deletion reduced MYC mRNA level. 2 independent pairs <t>of</t> <t>gRNAs</t> both decreased MYC mRNA by qRT-PCR normalized to b-actin. Control gRNA treated cells were set to 1. (D) Growth of MYCSE1 deleted LCLs and LCLs with deletion in a control region. (E) MYCSE2 deletion also reduced MYC mRNA level. 2 independent pairs of gRNAs both decreased MYC mRNA by qRT-PCR. All data are represented as mean+/−SE. See also Figure S3.
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    (A) RNAPII ChIA-PET linkages at the MYC locus. ChIP-seq tracks were shown on the top. MYCSE1 (-525kb from TSS) and MYCSE2 (-428kb from TSS) were indicated by red box below the tracks. RNAPII ChIA-PET links were shown by magenta lines. Orange boxes indicated the regions targeted by CRISPR. (B) Validation of MYCSE1 Deletion. Cas9 stable LCLs or BJAB were transduced with paired gRNA targeting the edges of ESE. After puromycin selection, genomic DNA was prepared from LCLs and BJAB cells transduced with dual gRNA. The targeted region was PCR amplified. The presence or absence of the MYC SE1 was shown. (C) MYCSE1 deletion reduced MYC mRNA level. 2 independent pairs of gRNAs both decreased MYC mRNA by qRT-PCR normalized to b-actin. Control gRNA treated cells were set to 1. (D) Growth of MYCSE1 deleted LCLs and LCLs with deletion in a control region. (E) MYCSE2 deletion also reduced MYC mRNA level. 2 independent pairs of gRNAs both decreased MYC mRNA by qRT-PCR. All data are represented as mean+/−SE. See also Figure S3.

    Journal: Cell host & microbe

    Article Title: The Epstein-Barr Virus Regulome in Lymphoblastoid Cells

    doi: 10.1016/j.chom.2017.09.001

    Figure Lengend Snippet: (A) RNAPII ChIA-PET linkages at the MYC locus. ChIP-seq tracks were shown on the top. MYCSE1 (-525kb from TSS) and MYCSE2 (-428kb from TSS) were indicated by red box below the tracks. RNAPII ChIA-PET links were shown by magenta lines. Orange boxes indicated the regions targeted by CRISPR. (B) Validation of MYCSE1 Deletion. Cas9 stable LCLs or BJAB were transduced with paired gRNA targeting the edges of ESE. After puromycin selection, genomic DNA was prepared from LCLs and BJAB cells transduced with dual gRNA. The targeted region was PCR amplified. The presence or absence of the MYC SE1 was shown. (C) MYCSE1 deletion reduced MYC mRNA level. 2 independent pairs of gRNAs both decreased MYC mRNA by qRT-PCR normalized to b-actin. Control gRNA treated cells were set to 1. (D) Growth of MYCSE1 deleted LCLs and LCLs with deletion in a control region. (E) MYCSE2 deletion also reduced MYC mRNA level. 2 independent pairs of gRNAs both decreased MYC mRNA by qRT-PCR. All data are represented as mean+/−SE. See also Figure S3.

    Article Snippet: Dual gRNAs were cloned into pLentiGuide-Puro (Addgene Plasmid #52963) using the Multiplex gRNA kit (System Biosciences) according to the manufacturer protocol.

    Techniques: ChIA Pet Assay, ChIP-sequencing, CRISPR, Biomarker Discovery, Transduction, Selection, Amplification, Quantitative RT-PCR, Control